Biological Nano-Objects -- Structural and Functional Regulation of DNA: Geometry, Topology and Methylation -- Protein#x2013;Lipid Assembly and Biomimetic Nanostructures -- Supramolecular Complexes of DNA -- Functionalised Inorganic Nanoparticles for Biomedical Applications -- Living Nanomachines -- Aptamer Selection by Darwinian Evolution -- Methods of Nanobiotechnology -- Optical Tools -- Nanoforce and Imaging -- Surface Methods -- Mass Spectrometry -- Electrical Characterisation and Dynamics of Transport -- Microfluidics: Concepts and Applications to the Life Sciences -- Data Processing -- Molecular Dynamics. Observing Matter in Motion -- Applications of Nanobiotechnology -- Real-Time PCR -- Biosensors. From the Glucose Electrode to the Biochip -- DNA Microarrays -- Protein Microarrays -- Cell Biochips -- Lab on a Chip -- Polyelectrolyte Multilayers -- Biointegrating Materials -- Viral Vectors for in Vivo Gene Transfer -- Pharmaceutical Applications of Nanoparticle Carriers -- Activatable Nanoparticles for Cancer Treatment. Nanobiotix -- The Medical, Social, and Economic Stakes of Nanobiotechnology.