In vitro chromatin assembly : strategies and quality control / Preparation, crystallization, and structure determination of chromatin enzyme/nucleosome complexes / Preparation of recombinant centromeric nucleosomes and formation of complexes with nonhistone centromere proteins / Functional characterization of histone chaperones using SNAP-tag-based imaging to assess De Novo histone deposition / Analysis of nucleosome sliding by ATP-dependent chromatin remodelling enzymes / In vitro activity assays for MYST histone acetyltransferases and adaptation for high-throughput inhibitor screening / Preparation and biochemical analysis of classical histone deacetylases / Recombinant preparation, biochemical analysis, and structure determination of sirtuin family histone/protein deacylases / Preparation, biochemical analysis, and structure determination of SET domain histone methyltransferases / Expression, purification, and biochemical analysis of the LSD1/KDM1A histone demethylase / LSD1 histone demethylase assays and inhibition / Purification, biochemical analysis, and structure determination of JmjC lysine demethylases / Preparation and analysis of native chromatin-modifying complexes / Preparation, biochemical analysis, and structure determination of the bromodomain, an acetyl-lysine binding domain / Preparation, biochemical analysis, and structure determination of methyllysine readers / Quantification of oxidized 5-methylcytosine bases and TET enzyme activity / Characterization of how DNA modifications affect DNA binding by C2H2 zinc finger proteins / Crystallographic studies of telomerase / Detection and analysis of long noncoding RNAs / Identifying centromeric RNAs involved in histone dynamics in vivo