CRISPR in animals and animal models

Title:

ISBN:

9780128125076

Publication Information New:

London : Academic Press, an imprint of Elsevier, 2017.

Physical Description:

1 online resource

Series:

Progress in molecular biology and translational science ; volume 152

Progress in molecular biology and translational science ; volume 152.

Contents:

Cover -- Title page -- Copyright -- Contents -- Contributors -- Chapter One -- CRISPR History: Discovery, Characterization, and Prosperity -- Abstract -- 1 Introduction -- 2 Discovery of CRISPR-Cas systems -- 2.1 Identification of CRISPR Loci and cas Genes -- 2.2 Prediction of Small RNA-Based Antiviral Functions of CRISPR -- 2.2.1 Identification of CRISPR-Related Small RNAs -- 2.2.2 Origin of Spacers in CRISPR Loci -- 2.3 Experimental Demonstration of the Basic Mechanism of CRISPR-Cas System -- 2.3.1 Adaptation -- 2.3.2 crRNA Biogenesis

2.3.3 Interference3 Molecular mechanisms of nucleic acid interference -- 3.1 PAM-Dependent DNA Interference by Type I CRISPR-Cas Systems -- 3.2 Type II CRISPR-Cas System Requires a Unique tracrRNA for DNA Interference -- 3.3 RNA-Activated DNA Interference by Type III CRISPR-Cas Systems -- 4 Discovery of anti-CRISPR system -- 5 Identification of novel CRISPR-Cas systems -- 6 Blooming of CRISPR technology -- References -- Chapter Two -- CRISPR/Cas9 Technology: Applications and Human Disease Modeling -- Abstract -- 1 Genome engineering tools

1.1 Genome Engineering Technologies1.1.1 Homologous Recombination (HR) -- 1.1.2 Double-Strand Breaks (DSBs) -- 1.1.3 NHEJ Versus HDR -- 1.1.4 Zinc Finger Nuclease (ZFN) -- 1.1.5 Transcription Activator-Like Effector Nucleases (TALENs) -- 1.1.6 CRISPR/Cas9 -- 1.2 Off Target Effects -- 2 CRISPR applications -- 2.1 CRISPRi and CRISPRa: Regulation of Transcriptional Levels -- 2.2 Epigenome Edition -- 2.3 Genome Imaging -- 2.4 Large-Scale Functional Genomic Studies Using CRISPR/Cas9 -- 2.5 Edition of Single-Stranded RNA

3 CRISPR applications in biomedical modeling3.1 Neurological Disease Models -- 3.2 Cancer Models -- 3.3 Cardiovascular Disease Models -- 3.4 Infectious Disease Models -- 3.5 Immunodeficiency Models -- 4 Concluding remarks -- References -- Chapter Three -- Dynamics of Indel Profiles Induced by Various CRISPR/Cas9 Delivery Methods -- Abstract -- 1 Introduction -- 1.1 Genome Editing and DNA Repair -- 1.2 Indel Detection Methodologies -- 1.3 IDAA for Indel Detection and gRNA Selection -- 2 Materials and methods -- 2.1 gRNAs, Plasmid Vectors and Cell Lines

2.2 Cas9 and gRNA Delivery Conditions2.3 Efficiency Measurements -- 3 Results -- 3.1 Rationale and Design of the Study -- 3.2 Transfection and Integration Dynamics -- 3.3 Efficiency of Generating Indels Over Time -- 3.4 Indel Profiles Over Time -- 3.5 Dynamic Effect of gRNA Stability on Indel Efficiency -- 3.6 Comparison of IDAA and TIDE Methods -- 4 Concluding remarks -- Acknowledgments -- References -- Chapter Four -- CRISPR Libraries and Screening -- Abstract -- 1 Introduction -- 2 Mouse models of cancer -- 3 CRISPR-Cas9 systems -- 4 CRISPR libraries

Abstract:

CRISPR in Animals and Animal Models, Volume 152, the latest release in the Progress in Molecular Biology and Translational Science series, explores the genome editing CRISPR system in cells and animal models, its applications, the uses of the CRISPR system, and the past, present and future of CRISPR genome editing. Topics of interest in this updated volume include a section on CRISPR history, The genome editing revolution, Programming CRISPR and its applications, CRISPR Delivery methods, CRISPR libraries and screening, CRISPR investigation in haploid cells, CRISPR in the generation of transgenic animals, CRISPR therapeutics, and Promising strategies and present challenges.

Local Note:

Elsevier

Subject Term:

Génétique moléculaire.

Génie génétique.

SCIENCE -- Chemistry -- Industrial & Technical.