How to Measure Load-Dependent Kinetics of Individual Motor Molecules Without a Force-Clamp; J. Sung, K.I. Mortensen, J.A. Spudich and H. Flyvbjerg Studying the Mechanochemistry of Processive Cytoskeletal Motors with an Optical Trap; V. Belyy and A. Yildiz Single-Molecule Optical Trapping Techniques to Study Molecular Mechanisms of a Replisome; B. Sun and M.D. Wang Recent Advances in Biological Single-Molecule Applications of Optical Tweezers and Fluorescence Microscopy; M.H. Shabestari, A.E.C. Meijering, W.H. Roos, G.J.L. Wuite and E.J.G. Peterman Direct Visualization of Helicase Dynamics Using Fluorescence Localization and Optical Trapping; C.-T. Lin and T. Ha High-Resolution Optical Tweezers Combined with Single-Molecule Confocal Microscopy; K.D. Whitley, M.J. Comstock and Y.R.

Chemla Integrating Optical Tweezers, DNA Tightropes and Single Molecule Fluorescence Imaging: Pitfalls and Traps; J. Wang, J.T. Barnett1, M.R. Pollard and N.M. Kad Single-Stranded DNA Curtains for Studying Homologous Recombination; C.J. Ma, J.B. Steinfeld and E.C. Greene Inserting Extrahelical Structures into Long DNA Substrates for Single-Molecule Studies of DNA Mismatch Repair; M.W. Brown, A.D.L. Torre and I.J. Finkelstein Single-Molecule Insight into Target Recognition by CRISPR-Cas Complexes; M. Rutkauskas, A. Krivoy, M. Szczelkun, C. Rouillon and R. Seidel Preparation of DNA Substrates and Functionalized Glass Surfaces for Correlative Nanomanipulation and Colocalization (NanoCOSM) of Single Molecules; C. Duboc, J. Fan, E.T. Graves and T.R.

Strick Measuring Force Induced Dissociation Kinetics of Protein Complexes Using Single Molecule Atomic Force Microscopy; K. Manibog, C.-F. Yen and S. Sivasankar Improved Force Spectroscopy Using Focused-Ion-Beam Modified Cantilevers; J.K. Faulk, D.T. Edwards, M.S. Bull and T.T. Perkins Single-Molecule Characterization of DNA-Protein Interactions Using Nanopore Biosensors; A.H. Squires, T. Gilboa, C. Torfstein, N. Varongchayakul and A. Meller Subangstrom Measurements of Enzyme Function using a Biological Nanopore, SPRNT; A.H. Laszlo, I.M. Derrrington and J.H. Gundlach Multiplexed, Tethered Particle Microscopy for Studies of DNA-Enzyme Dynamics; S. Ucuncuoglu, D.A. Schneider, E.R. Weeks, D. Dunlap and L. Finzi