Front Cover; Advances in Clinical Chemistry; Copyright; Contents; Contributors; Preface; Chapter One: Advances in Cardiac Biomarkers of Acute Coronary Syndrome; 1. Introduction; 1.1. Acute Coronary Syndrome; 1.2. Myocardial Infarction; 2. Evolution of the Criteria to Define Myocardial Infarction; 3. Troponin Structure, Function, and Circulating Isoforms; 4. Standardization of Troponin Assays; 5. Analytical Performance Characteristics and Terminology Related to Troponin Assays; 5.1. Limit of Blank, Limit of Detection, and Limit of Quantitation.

5.2. Analytical Sensitivity and Imprecision at the 99th Percentile URL5.3. Classification of cTn Assays; 6. Serial ``Delta ́́Troponin Changes; 7. Point of Care Troponin Assays; 7.1. Analytical Recommendations for POC cTn Assays; 8. Preanalytical Factors Influencing cTn Results; 8.1. Specimen Type; 8.2. Hemolysis; 8.3. Endogenous Interferences; 9. Defining Normality and the 99th Percentile URL; 9.1. Sex; 9.2. Age; 9.3. Surrogate Biomarkers; 9.4. Population Size; 9.5. Recommendations for Defining Normality; 10. High-Sensitivity Troponin and Advancements in the Diagnosis of AMI.

10.1. Accelerated Serial Sampling Protocols for Rapid Rule-In/Rule-Out10.2. Frequency of AMI Diagnosis; 11. High-Sensitivity Troponin Rule-Out Strategies; 11.1. Undetectable Baseline Troponin Concentrations; 11.2. Accelerated Diagnostic Protocols; 12. Cardiac Troponin: Risk Stratification and Prognosis in Various Populations; 12.1. Renal Disease; 12.2. Perioperative Setting; 13. Other Biomarkers in ACS; 13.1. Natriuretic Peptides; 13.2. Copeptin; 13.3. Soluble ST2; 14. Conclusions; References; Chapter Two: Vitamin D Testing-Where Are We and What Is on the Horizon?; 1. Introduction.

2. Measurement of Vitamin D Metabolites2.1. Measurement of 25OH Vitamin D; 2.1.1. Physical Detection Methods-25OH Vitamin D; 2.1.2. Immunoassays-25OH Vitamin D; 2.2. Measurement of 1,25(OH)2 Vitamin D; 2.2.1. Physical Detection Methods-1,25(OH)2 Vitamin D; 2.2.2. Immunoassays-1,25(OH)2 Vitamin D; 2.3. Free and Bioavailable 25OH Vitamin D; 2.4. 24,25(OH)2 Vitamin D; 2.5. C3-epi-25OH Vitamin D; 3. Future Directions; 3.1. Improvement of Existing Methods; 3.2. Novel Assays; 4. Conclusion; References; Chapter Three: Urine Exosomes: An Emerging Trove of Biomarkers; 1. Introduction.

2. Composition and Biogenesis3. Candidate Biomarkers; 4. Methodology; 4.1. Storage and Processing; 4.2. Isolation; 4.2.1. Ultracentrifugation; 4.2.2. Filtration; 4.2.3. Precipitation; 4.2.4. Affinity Purification; 4.2.5. Microfluidics; 4.3. Quantification; 4.4. Constituent Analysis; 5. Barriers; 6. Conclusion; Acknowledgments; References; Chapter Four: Identification of Genomic Somatic Variants in Cancer: From Discovery to Actionability; 1. Introduction; 2. Current Sequencing Platforms and Assay Design; 2.1. Identification of Variants.