An increasing population has put tremendous pressure on agricultural productivity to fulfill the demands of human consumption. Numerous agricultural activities and techniques have been developed to raise annual crop production globally. While agriculture has succeeded in enhancing the yearly crop productivity, this achievement is at the cost of environmental degradation by applying synthetic persistent substances, such as industrial fertilizers, pesticides, herbicides, etc. Chemical fertilizers are nearly as destructive as they are productive, causing monocultures and consequences associated with elimination of diversity, nutrient pollution as evidenced by algae blooms, eutrophication, water quality issues, lower oxygen levels and dangers to fish stocks. Therefore, the scientific approach to maintain sustainable fertility in soil and plants is to switch over to biofertilisers. Biofertilisers are compounds of organic matter that are applied to crops for growth and health. Their constituent micro-organisms interact in an ecofriendly manner with the soil, root and seeds of plants, promoting the growth of micro-flora that enhances soil fertility. They are known to play a number of vital roles in soil fertility, crop productivity and production in agriculture. Application of biofertilisers results in increased mineral and water uptake, root development, vegetative growth and nitrogen fixation. They liberate growth promoting substances and vitamins and help to maintain soil fertility. They act as antagonists and play a pivotal role in neutralising the soil borne plant pathogens, thereby assisting in the bio-control of diseases. Application of biofertilisers in lieu of synthetic fertilizers could be the promising technique to raise agricultural productivity without degrading the environmental quality. The present book focuses on the latest research approaches and updates from the microbiota ecosystem and their applications in agriculture industry. It also highlights the great potential and possible future of action of microbiota in the development of sustainable agricultural systems.