This book constitutes the thoroughly refereed post-conference proceedings of the 16th International Conference on Smart Card Research and Advanced Applications, CARDIS 2017, held in Lugano, Switzerland, in November 2017. The 14 revised full papers presented together with 2 abstracts of invited talks in this book were carefully reviewed and selected from 48 submissions.  CARDIS has provided a space for security experts from industry and academia to exchange on security of smart cards and related applications.