This book constitutes the thoroughly refereed post-conference proceedings of the 13th International Conference on Artificial Evolution, EA 2017, held in Paris, France, in October 2017. The 16 revised papers were carefully reviewed and selected from 33 submissions. The papers cover a wide range of topics in the field of artificial evolution, such as evolutionary computation, evolutionary optimization, co-evolution, artificial life, population dynamics, theory, algorithmics and modeling, implementations, application of evolutionary paradigms to the real world (industry, biosciences, ...), other biologically-inspired paradigms (swarm, artificial ants, artificial immune systems, cultural algorithms...), memetic algorithms, multi-objective optimisation, constraint handling, parallel algorithms,, dynamic optimization, machine learning and hybridization with other soft computing techniques.