Journey of Human Gametes In Vitro: 1978 to 2010 -- Building the Laboratory -- Air Quality Management -- Organizational Aspects of the Laboratory in a Tertiary Care ART Center -- Quality Control Management -- Daily, Weekly, and Regular Preparations for the IVF Laboratory -- Risk and Safety in the IVF Clinic -- Essential Instruments and Disposable Supplies for an IVF Laboratory -- Co2 and Low-O2 Incubators -- IVF Workstations -- Culture Media in IVF: Decisions for the Laboratory -- Oocyte Denuding -- Assessment of Oocyte Quality -- Polarization Microscopy -- Cumulus Cell Gene Expression in Assessment of Oocyte Quality -- Short Culture: Day 1/Day 2/Day 3 Embryo Culture -- Extended Culture in IVF -- In Vitro Maturation of Human Oocytes -- In Vivo Embryo Culture Device -- Microfluidics for Gamete Manipulation and Embryo Culture -- Sperm Assessment: Traditional Approaches and Their Indicative Value -- Sperm Assessment: Novel Approaches and Their Indicative Value -- Sperm Processing for IVF -- PESA/TESA/TESE Sperm Processing -- Processing Sperm Samples in HIV-Positive Patients -- Intracytoplasmic Morphologically Selected Sperm Injection -- Sperm Testing and ICSI Selection by Hyaluronic Acid Binding: The Hyaluronic Acid-Coated Glass Slide and Petri Dish in the Andrology and IVF Laboratories.-  Electrophoretic Sperm Separation -- Magnetic Activated Cell Sorting of Human Spermatozoa -- Poloscope-Based Sperm Selection -- Intrauterine Insemination -- Conventional IVF Insemination -- Intracytoplasmic Sperm Injection -- Mechanism of Human Oocyte Activation During ICSI and Methodology for Overcoming Low or Failed Fertilization -- Hydraulic Manipulators for ICSI -- Research Instruments Micromanipulators -- Eppendorf Micromanipulator: Setup and Operation of Electronic Micromanipulators -- The Leica-IMSI System -- Automated Robotic Intracytoplasmic Sperm Injection -- Oocyte Treatment and Preparation for Microinjection -- Livestock Production Via Micromanipulation -- Pronuclear Scoring in Human In Vitro Fertilization -- Cumulative Morphological Assessment of Embryo Quality -- Metabolomics: The ViaMetrics-E™ Procedure for Assessing Embryo Viability -- Oxygen Consumption as an Indicator of Oocyte and Embryo Viability -- Gene Expression Changes During Human Early Embryo Development: New Applications for Embryo Selection -- Amino Acid Turnover as a Biomarker of Embryo Viability -- Real-time Embryo Monitoring Devices for Embryo Selection -- Assisted Hatching in IVF -- Polar Body Biopsy -- Cleavage Stage Embryo Biopsy -- Embryo Biopsy for PGD: Current Perspective -- Microarrays and CGH for PGD of Chromosome Abnormalities and Gene Defects -- Sperm Cryopreservation -- Slow Freezing of Oocytes -- Vitrification: Research in Animal Models -- Oocyte Vitrification -- Slow Freezing of Embryos -- Vitrification of Embryos -- Directional Freezing of Reproduction Cells, Tissues, and Organs -- Ovarian Tissue Cryopreservation -- Assessment of Uterine Receptivity -- Single Embryo Transfer -- Ultrasound-Guided Embryo Transfer (Abdominal/Vaginal): An Evidence-Based Evaluation -- Cumulus-Aided Embryo Transfer -- Data Management in the ART Laboratory: Requirements and Solutions -- Regulation, Licensing, and Accreditation of the ART Laboratory -- Legislation in the United Kingdom -- Regulation, Licensing, and Accreditation of the ART Laboratory in Europe -- Regulation, Licensing, and Accreditation of ART Laboratories in India -- Troubleshooting in the Clinical Embryology Laboratory: The Art of Problem-Solving in ART -- The Role of Mitochondria in the Establishment of Developmental Competence in Early Human Development -- Nuclear and Cytoplasmic Transfer: Human Applications and Concerns -- Cytoskeletal Architecture of Human Oocytes with Focus on Centrosomes and Their Significant Role in Fertilization -- Molecular Mining of Follicular Fluid for Reliable Biomarkers of Human Oocyte and Embryo Developmental Competence.