Front Cover; Series Editors; Laboratory Methods in Cell Biology: Biochemistry and Cell Culture; Copyright; Contents; Contributors; Preface: Laboratory Methods in CellBiology; CHAPTER1 -- Extraction/Characterization of DNA; 1 PURPOSE; 2 THEORY; 3 EQUIPMENT (IN ORDER OF APPEARANCE); 4 MATERIALS (IN ORDER OF APPEARANCE); 5 PROTOCOL; CHAPTER2 -- CO-IMMUNOPRECIPITATION: ISOLATION OF PROTEIN SIGNALING COMPLEXES FROM NATIVE TISSUES; 1 PURPOSE; 2 THEORY; 3 EQUIPMENT; 4 MATERIALS; 5 PROTOCOL; References; CHAPTER 3 -- REAL-TIME PCR; 1 PURPOSE; 2 THEORY; 3 EQUIPMENTS; 4 MATERIALS; 5 PREPARE WORKSPACE

6 PROTOCOLReferences; CHAPTER4 -- SINGLE-CELL GEL ELECTROPHORESIS COMBINED WITH LESION-SPECIFIC ENZYMES TO MEASURE OXIDATIVE DAMAGE TO DNA; 1 PURPOSE; 2 THEORY; 3 EQUIPMENTS; 4 MATERIALS; 5 PROTOCOL; References; CHAPTER5 -- HIGH-THROUGHPUT METHODS FOR THE IDENTIFICATION OF PROTEIN PURIFICATION CONDITIONS USING A CLEAVABLE TAG SYSTEM; 1 PURPOSE; 2 THEORY; 3 EQUIPMENT; 4 MATERIALS; 5 PROTOCOL; References; CHAPTER6 -- TWO-DIMENSIONAL DIFFERENCE GEL ELECTROPHORESIS (2D DIGE); 1 PURPOSE; 2 THEORY; 3 EQUIPMENT; 4 MATERIALS; 5 PROTOCOL; References

CHAPTER7 -- OPTIMIZATION OF CELL CYCLE MEASUREMENT BY TIME-LAPSE MICROSCOPY1 PURPOSE; 2 THEORY; 3 DETERMINATION OF CELL CYCLE LENGTH; 4 EQUIPMENTS; 5 MATERIALS; 6 PROTOCOL; Video; References; CHAPTER8 -- TRANSFECTION METHODS; 1 PURPOSE; 2 THEORY; 3 EQUIPMENT; 4 MATERIALS; 5 PROTOCOL; References; CHAPTER 9 -- CELL ENRICHMENT FROM HUMAN BLOOD THROUGH RED CELL LYSIS; 1 PURPOSE; 2 THEORY; 3 EQUIPMENTS; 4 MATERIALS; 5 PROTOCOL; References; CHAPTER10 -- MAGNETIC, BATCH SEPARATION; 1 PURPOSE; 2 THEORY; 3 EQUIPMENT; 4 MATERIALS; 5 PROTOCOL; References

CHAPTER11 -- LATERAL FLOW SANDWICH ASSAY UTILIZING UPCONVERTING PHOSPHOR (UCP) REPORTERS1 PURPOSE; 2 THEORY; 3 EQUIPMENTS; 4 MATERIALS; 5 PROTOCOL; References; CHAPTER 12 -- IN VIVO MODULATION OF MICRORNA LEVELS DURING SPINAL CORD REGENERATION; 1 PURPOSE; 2 THEORY; 3 EQUIPMENTS; 4 MATERIALS; 5 PROTOCOL; References; CHAPTER 13 -- COMETCHIP: SINGLE-CELL MICROARRAY FOR HIGH-THROUGHPUT DETECTION OF DNA DAMAGE; 1 PURPOSE; 2 THEORY; 3 EQUIPMENTS; 4 MATERIALS; 5 PROTOCOL; References; CHAPTER 14 -- PURIFICATION OF CARDIAC CELLS; 1 THEORY; 2 EQUIPMENTS; 3 MATERIALS; 4 PROTOCOL; Reference

CHAPTER 15 -- TUBE FORMATION IN VITRO ANGIOGENESIS ASSAY1 PURPOSE; 2 THEORY; 3 EQUIPMENTS; 4 MATERIALS; 5 PROTOCOL; References; CHAPTER 16 -- PROCESSING OF TRANSMISSION ELECTRON MICROSCOPY IMAGES FOR SINGLE-PARTICLE ANALYSIS OF MACROMOLECULAR COMPLEXES; 1 PURPOSE; 2 THEORY; 3 EQUIPMENT; 4 MATERIALS; 5 PROTOCOL; References; CHAPTER 17 -- AN ASYMMETRIC 3D IN VITRO ASSAY FOR THE STUDY OF TUMOR CELL INVASION; 1 PURPOSE; 2 THEORY; 3 EQUIPMENT; 4 MATERIALS; 5 PROTOCOL; References; CHAPTER 18 -- ISOLATION OF MYOBLASTS FROM MUSCLE TISSUE BY PREPLATING; 1 PURPOSE; 2 THEORY; 3 EQUIPMENT; 4 MATERIALS