1. The chemistry of small-molecule fluorogenic probes / Jonathan B. Grimm, Laurel M. Heckman, and Luke D. Lavis -- 2. Fluorescent environment-sensitive dyes as reporters of biomolecular interactions / Andrey S. Klymchenko and Yves Mely -- 3. Rational design of fluorophores for in vivo applications / Marcin Ptaszek -- 4. Fluorescence technologies for monitoring interactions between biological molecules in vitro / Sebastien Deshayes and Gilles Divita -- 5. From FRET imaging to practical methodology for kinase activity sensing in living cells / Francois Sipieter [and eight others] -- 6. Fluorescent sensors of protein kinases : from basics to biomedical applications / Thi Nhu Ngoc Van and May C. Morris -- 7. Time-resolved Forster resonance energy transfer-based technologies to investigate G protein-coupled receptor machinery : high-throughput screening assays and future development / Pauline Scholler [and six others] -- 8. Fluorescent protein-based biosensors and their clinical applications / Yusuke Ohba [and three others] -- 9. Fluorescent macromolecular sensors of enzymatic activity for in vivo imaging / Alexei A. Bogdanov and Mary L. Mazzanti -- 10. Fluorescent proteins as visible in vivo sensors / Robert M. Hoffman.