Chapter One. RNA Imaging with Multiplexed Error-Robust Fluorescence In Situ Hybridization (MERFISH) / J.R. Moffitt, X. Zhuang -- Chapter Two. Imaging Single mRNA Dynamics in Live Neurons and Brains / H.C. Moon, H.Y. Park -- Chapter Three. Monitoring of RNA Dynamics in Living Cells Using PUM-HD and Fluorescent Protein Reconstitution Technique / H. Yoshimura, T. Ozawa -- Chapter Four. Applications of Hairpin DNA-Functionalized Gold Nanoparticles for Imaging mRNA in Living Cells / S.R. Jackson, A.C. Wong, A.R. Travis, I.E. Catrina, D.P. Bratu, D.W. Wright, A. Jayagopal -- Chapter Five. In Vivo RNA Visualization in Plants Using MS2 Tagging / E.J. Peña, M. Heinlein -- Chapter Six. TRICK: A Single-Molecule Method for Imaging the First Round of Translation in Living Cells and Animals / J.M. Halstead, J.H. Wilbertz, F. Wippich, T. Lionnet, A. Ephrussi, J.A. Chao -- Chapter Seven. Fluctuation Analysis: Dissecting Transcriptional Kinetics with Signal Theory / A. Coulon, D.R. Larson -- Chapter Eight. IMAGEtags: Quantifying mRNA Transcription in Real Time with Multiaptamer Reporters / J. Ray, I. Shin, M. Ilgu, L. Bendickson, V. Gupta, G.A. Kraus, M. Nilsen-Hamilton -- Chapter Nine. A Method for Expressing and Imaging Abundant, Stable, Circular RNAs In Vivo Using tRNA Splicing / C.A. Schmidt, J.J. Noto, G.S. Filonov, A.G. Matera -- Chapter Ten. RNA-ID, a Powerful Tool for Identifying and Characterizing Regulatory Sequences / C.E. Brule, K.M. Dean, E.J. Grayhack -- Chapter Eleven. Fluorescent Protein-Based Quantification of Alternative Splicing of a Target Cassette Exon in Mammalian Cells / N.G. Gurskaya, D.B. Staroverov, K.A. Lukyanov -- Chapter Twelve. IRAS: High-Throughput Identification of Novel Alternative Splicing Regulators / S. Zheng -- Chapter Thirteen. Analysis of Nonsense-Mediated mRNA Decay at the Single-Cell Level Using Two Fluorescent Proteins / N.G. Gurskaya, A.P. Pereverzev, D.B. Staroverov, N.M. Markina, K.A. Lukyanov -- Chapter Fourteen. Developing Fluorogenic Riboswitches for Imaging Metabolite Concentration Dynamics in Bacterial Cells / J.L. Litke, M. You, S.R. Jaffrey.