Recent advances in development of genetically encoded fluorescent sensors -- Engineering rugged field assays to detect hazardous chemicals using spore-based bacterial biosensors -- Engineering BRET-sensor proteins -- Enzymes as sensors -- Integrated strategies to gain a systems-level view of dynamic signaling networks -- Probing Cdc42 polarization dynamics in budding yeast using a biosensor -- Novel fluorescence-based biosensors incorporating unnatural amino acids -- Folding- and dynamics-based electrochemical DNA sensors -- Construction of protein-based biosensors using ligand-directed chemistry for detecting analyte binding -- Measuring and imaging metal ions with fluorescence-based biosensors: speciation, selectivity, kinetics, and other issues -- Bioapplications of electrochemical sensors and biosensors -- A highly sensitive biosensor for ATP using a chimeric firefly luciferase -- Highly modular bioluminescent sensors for small molecules and proteins -- Sensitive protein detection and quantification in paper-based microfluidics for the point of care -- Microneedle enzyme sensor arrays for continuous in vivo monitoring -- Visualization of the genomic loci that are bound by specific multiprotein complexes by biomolecular fluorescence complementation analysis on Drosophila polytene chromosomes -- Author index -- Subject index.