Correlative light and electron microscopy III
Başlık:
Correlative light and electron microscopy III
ISBN:
9780128099766
Yayın Bilgileri:
Amsterdam, Netherlands ; Boston, Mass. : Elsevier / Academic Press, [2017]
©2017
Fiziksel Tanımlama:
1 online resource (xvii, 352 pages) : illustrations (some color)
Series:
Methods in cell biology, volume 140
Methods in cell biology ; v. 140.
Contents:
Front Cover; Methods in Cell Biology; Series Editors; Methods in CellBiology: Correlative Light and Electron Microscopy III; Copyright; Contents; Contributors; Preface: The Key Lies in the Right Combination; 1 -- Millisecond time resolution correlative light and electron microscopy for dynamic cellular processes; INTRODUCTION; 1. METHODS; 1.1 Chlamydomonas reinhardtii Culture; 1.2 Imaging and Embedding Chamber Preparation; 1.3 Light Microscopy; 1.4 Sample Preparation for Electron Microscopy; 1.4.1 Chemical postfixation and embedding; 1.4.2 Thin sectioning
1.4.3 Staining of the sections and application of fiducial markers1.5 Electron Tomography; 1.6 Registration of Light and Electron Microscopy Images; 1.7 Data Analysis; 1.8 Preparation of Flagellar Cross Sections for Correlative Light and Electron Microscopy Analysis; 2. INSTRUMENTATION AND MATERIALS; 2.1 Chlamydomonas reinhardtii culture; 2.2 Imaging and Embedding Chamber Preparation; 2.3 Light Microscopy; 2.4 Sample Preparation for Electron Microscopy; 2.4.1 Chemical postfixation and embedding; 2.4.2 Thin sectioning; 2.4.3 Staining of the sections and application of fiducial markers
2.5 Electron Tomography2.6 Registration of Light and Electron Microscopy Images; 2.7 Data Analysis; 2.8 Preparation of Flagellar Cross Sections for Correlative Light and Electron Microscopy Analysis; 3. DISCUSSION AND OUTLOOK; Acknowledgments; REFERENCES; 2 -- 3D subcellular localization with superresolution array tomography on ultrathin sections of various species; INTRODUCTION AND RATIONALE; 1. METHODS-CORE PROTOCOL; 1.1 High-Pressure Freezing; 1.2 Freeze Substitution; 1.3 Embedding; 1.4 Ultramicrotomy; 1.5 Light Microscopy; 1.6 Contrasting and Carbon Coating
1.7 Scanning Electron Microscopy1.8 Image Processing and Correlation of Structured Illumination Microscopy and Scanning Electron Microscopy Images; 1.9 Alignment and 3D Reconstruction; 1.9.1 IMOD; 1.9.2 Fiji and AMIRA; 2. METHODS-MODEL-SPECIFIC ADAPTATIONS AND CONSIDERATIONS; 2.1 Caenorhabditis elegans; 2.2 Trypanosoma brucei; 2.3 Social Insects (Apis mellifera and Cataglyphis fortis); 3. INSTRUMENTATION AND MATERIALS; 3.1 High-Pressure Freezing; 3.1.1 General; 3.1.2 Caenorhabditis elegans; 3.1.3 Trypanosoma brucei; 3.1.4 Cataglyphis fortis and Apis mellifera; 3.2 Freeze Substitution
3.3 Embedding3.4 Ultramicrotomy; 3.5 Light Microscopy; 3.5.1 Primary Antibodies; 3.6 Contrasting and Carbon Coating; 3.7 Scanning Electron Microscopy; 3.8 Image Processing and Correlation of Structured Illumination Microscopy and Scanning Electron Microscopy Images; 3.9 Alignment and 3D Reconstruction; CONCLUSIONS; Acknowledgments; REFERENCES; 3 -- Preserving the photoswitching ability of standard fluorescent proteins for correlative in-resin super-resolutio ... ; INTRODUCTION; 1. RATIONALE; 2. MATERIALS; 2.1 Instrumentation; 2.2 Materials; 2.3 Chemicals; 3. METHODS; 3.1 Reagent Preparation
Abstract:
The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron microscopy; CLEM has been employed for decades to generate molecular identification that can be visualized by a dark, electron-dense precipitate. This new volume of Methods in Cell Biology covers many areas of CLEM including a brief history and overview on CLEM methods, imaging of intermediate stages of meiotic spindle assembly in C. elegans embryos using CLEM, and capturing endocytic segregation events with HPF-CLEM. Covers many areas of CLEM by the best international scientists in the field. Includes a brief history and overview on CLEM methods.
Local Note:
Elsevier
Elektronik Erişim:
Full Text Available From Elsevier e-Books
Dil:
English