Structural and mechanistic enzymology
Başlık:
Structural and mechanistic enzymology
ISBN:
9780128118771
Edition:
First edition.
Yayın Bilgileri:
Cambridge, MA : Academic Press is an imprint of Elsevier, 2017.
Fiziksel Tanımlama:
1 online resource
Series:
Advances in protein chemistry and structural biology ; volume 109
Advances in protein chemistry and structural biology ; v. 109.
Contents:
Title page; Table of Contents; Copyright; Contributors; Preface; Chapter One: Collagenolytic Matrix Metalloproteinase Structure-Function Relationships: Insights From Molecular Dynamics Studies; Abstract; 1 Matrix Metalloproteinases; 2 MD Simulation Approaches; 3 MD Simulation of the Collagen Triple Helix; 4 MD Simulation of the MMP Cleavage Site in Collagen; 5 MD Consideration of Water Surrounding Collagen; 6 MD of MMP-1 or MMP-8 Interaction With Collagen; 7 MD of MMP-2 Interaction With Collagen; 8 Mechanism of Collagenolysis; Acknowledgments
Chapter Two: Computational Glycobiology: Mechanistic Studies of Carbohydrate-Active Enzymes and Implication for Inhibitor DesignAbstract; 1 Introduction; 2 Computational Methods; 3 Puckering of Carbohydrate Structures; 4 Identification of Catalytically Competent Protonation States of Key Residues; 5 Clarification of Catalytic Mechanisms; 6 Transition-State Analogues as Inhibitors of CAZymes; 7 Conclusions and Future Directions; Acknowledgments; Chapter Three: Computational Biochemistry-Enzyme Mechanisms Explored; Abstract; 1 Introduction; 2 Structural Models
3 Calculating Enzymatic Mechanisms4 Going Beyond the Exploration of the Reaction Paths; Chapter Four: A Paradigm for C-H Bond Cleavage: Structural and Functional Aspects of Transition State Stabilization by Mandelate Racemase; Abstract; 1 Introduction; 2 Structure and Function Within the MR Subgroup; 3 MR Catalysis: An Uphill Battle; 4 Meeting the Challenges: Solutions Afforded by MR; 5 Mandelate Racemase Structure: Ligand Binding Within a Catalytic Cage; 6 Evolutionary Relationships: A Mechanistic Link to Aldolases?; 7 Concluding Remarks; Acknowledgments
Chapter Five: Striking Diversity in Holoenzyme Architecture and Extensive Conformational Variability in Biotin-Dependent CarboxylasesAbstract; 1 Introduction; 2 Structures of Conserved Components in Biotin-Dependent Carboxylases; 3 Extensive Diversity in Holoenzyme Architectures; 4 Extensive Conformational Variability; 5 Perspectives; Acknowledgments; Chapter Six: Enzymology of Microbial Dimethylsulfoniopropionate Catabolism; Abstract; 1 Introduction; 2 DMSP Demethylation Pathway and the Demethylase, DmdA; 3 DMSP Cleavage Enzymes
4 The CoA-Transferase Pathway of DMS and 3-Hydroxypropionate Formation Using DddD5 The Lyase Pathway of DMS and Acrylate Generation; 6 Summary and Perspective; Acknowledgments; Chapter Seven: Sortase Transpeptidases: Structural Biology and Catalytic Mechanism; Abstract; 1 Introduction; 2 Structural Biology: Enzyme Structure and Class-Specific Variations; 3 Structural Biology: Molecular Basis of Substrate Recognition; 4 Computational Studies; 5 Catalytic Mechanism; 6 In Vitro Transpeptidation Activity; 7 Conclusions and Future Directions
Abstract:
"Structural and Mechanistic Enzymology, Volume 109, the latest release in the Advances in Protein Chemistry and Structural Biology series, is an essential resource for protein chemists. Its volumes bring forth new information about protocols and analysis of proteins, with each thematically organized volume guest edited by leading experts in a broad range of protein-related topics. This volume presents state-of-the-art contributions, providing insights into the relationship between enzyme structure, catalysis, and function. The understanding of enzymatic molecular mechanisms requires a combined application of a broad range of experimental and computational techniques. Experimental methods such as NMR spectroscopy, X-ray crystallography, molecular biology, and kinetic studies, combined with a range of molecular modeling techniques such as molecular dynamics, quantum chemistry, and docking, provide us a synergistic approach for gaining insights into enzyme catalysis, structure, and function. Provides cutting-edge developments in protein chemistry and structural biologyFeatures new information about protocols and analysis of proteinsContains chapters written by authorities in their respective fieldsTargeted to a wide audience of researchers, specialists and students"-- Provided by publisher.
Local Note:
Elsevier
Added Author:
Elektronik Erişim:
Full Text Available From Elsevier e-Books
Dil:
English