Title page; Table of Contents; Copyright; Contributors; Preface; Chapter One: Genome Engineering and Agriculture: Opportunities and Challenges; Abstract; 1 Introduction; 2 Genome Editing in Plants: Potential DNA Modifications; 3 Agricultural Demands and Genome Editing Successes; 4 Creating Healthier and More Nutritious Food; 5 Challenges and Future Outlook; 6 Concluding Thoughts; Chapter Two: Use of CRISPR/Cas9 for Crop Improvement in Maize and Soybean; Abstract; 1 Introduction; 2 Examples of CRISPR/Cas for Crop Improvement in Maize; 3 Examples of CRISPR/Cas for Crop Improvement in Soybean

4 Looking ForwardAcknowledgments; Chapter Three: Use of Zinc-Finger Nucleases for Crop Improvement; Abstract; 1 Introduction; 2 Why Use ZFNs?; 3 Plant Genome Editing Using ZFNs; 4 Targeted Gene Editing; 5 Enhancing the Efficiency of Genome Editing; 6 Regulatory Framework; 7 Concluding Statements; Chapter Four: Gene Editing in Polyploid Crops: Wheat, Camelina, Canola, Potato, Cotton, Peanut, Sugar Cane, and Citrus; Abstract; 1 Introduction; 2 Gene Editing in Polyploid Crops; Chapter Five: Gene Editing With TALEN and CRISPR/Cas in Rice; Abstract; 1 Introduction

2 Creation of DNA Double-Strand Breaks to Allow Genome Editing3 Use of TALENs for Gene Editing in Rice; 4 Use of CRISPR/Cas9 for Gene Editing in Rice; 5 Use of CRISPR/Cpf1 for Gene Editing in Rice; 6 Use of Base Editor in Rice; 7 Concluding Remarks; Chapter Six: Genome Editing to Improve Abiotic Stress Responses in Plants; Abstract; 1 Introduction; 2 Optimization of CRISPR/Cas9 System to Generate Mutant Plants; 3 Developing a New Allele of a Plant Abiotic Stress-Responsive Gene; 4 Development of New Gene Delivery Systems for CRISPR/Cas9; Acknowledgments

Chapter Seven: CRISPR/Cas9-Enabled Multiplex Genome Editing and Its ApplicationAbstract; 1 Introduction; 2 Strategies to Achieve CRISPR/Cas9-Enabled Multiplex Genome Editing; 3 Broad Applications of Multiplex Genome Editing in Plants; 4 Conclusion; Acknowledgments; Chapter Eight: CRISPR/Cas9-Based Genome Editing in Plants; Abstract; 1 Introduction; 2 The Mechanism of the CRISPR/Cas9 Nuclease System; 3 Analysis of Targeted Mutations Induced by CRISPR/Cas9 in Plants; 4 Applications of CRISPR/Cas9 System for Plant Functional Studies and Crop Improvement; 5 Concluding Remarks and Perspectives

Chapter Nine: On Improving CRISPR for Editing Plant Genes: Ribozyme-Mediated Guide RNA Production and Fluorescence-Based Technology for Isolating Transgene-Free Mutants Generated by CRISPRAbstract; 1 Production of Cas9 Protein in Plant Cells; 2 Production of sgRNA Molecules In Vivo; 3 Production of sgRNAs From Ribozyme-Flanked Artificial Gene; 4 Efficient Identification of Cas9-Free Plants With the Desired Editing Events; Acknowledgment; Chapter Ten: Engineering Molecular Immunity Against Plant Viruses; Abstract; 1 Introduction; 2 The Use of SSNs for Genome Engineering