A structure-function toolbox for membrane transporter and channels

Başlık:

ISBN:

9780128123546

Edition:

First edition

Yayın Bilgileri:

Cambridge, MA : Academic Press is an imprint of Elsevier, 2017.

Fiziksel Tanımlama:

1 online resource (xii, 289 pages) : illustrations (some color)

Series:

Methods in enzymology ; v. 594

Methods in enzymology ; v. 594.

Genel Not:

Includes indexes.

Contents:

Chapter 1: Lipid Nanodiscs as a Tool for High-Resolution Structure Determination of Membrane Proteins by Single-Particle Cryo-EM / Rouslan Efremov, Christos Gatsogiannis and Stefan Raunser -- Chapter 2: SSM-Based Electrophysiology for Transporter Research / Andre Bazzone, Maria Barthmes and Klaus Fendler -- Chapter 3: Saposin-Lipoprotein Scaffolds for Structure Determination of Membrane Transporters / Joseph Lyons, Andreas Bøggild, Poul Nissen and Jens Frauenfeld -- Chapter 4: Single-Molecule Fluorescence Studies of Membrane Transporters Using Total Internal Reflection Microscopy / Joris M.H. Goudsmits, Antoine M. van Oijen and Dirk J. Slotboom -- Chapter 5: The MEMbrane Protein Single ShoT Amplification Recipe: MemStar / Aziz Abdul-Qureshi, Pascal F. Meier, Chiara Lee and David Drew -- Chapter 6: Efficient Screening and Optimization of Membrane Protein Production in Escherichia coli / Jacopo Marino, Katharina Holzhüter, Benedikt Kuhn and Eric R. Geertsma -- Chapter 7: Pinning Down the Mechanism of Transport: Probing the Structure and Function of Transporters Using Cysteine Cross-Linking and Site-Specific Labeling / Christopher Mulligan and Joseph A. Mindell -- Chapter 8: Ion Channel Conformation and Oligomerization Assessment by Site-Directed Spin Labeling and Pulsed-EPR / Christos Pliotas -- Chapter 9: Strategy for the Thermostabilization of an Agonist-Bound GPCR Coupled to a G Protein / Annette Strege, Byron Carpenter, Patricia C. Edwards and Christopher G. Tate.

Abstract:

New chapters in the updated serial include Cryo-EM on membrane proteins embedded in nanodics, Solid-Supported membrane-based electrophysiology on membrane transporters and channels, Saposin-lipoprotein scaffolds for structure determination of membrane transporters and channels, Single-molecule FRET on transporters, Dynamics of channels and transporters investigated by NMR, Structure-function studies on channels and transporters, and a section on MemStar, a new GFP-based expression and purification system for transporters and channels.

Local Note:

Elsevier

Subject Term:

Biological transport.

Transport biologique.

Protéines membranaires.

Canaux ioniques.