Using the Flp recombinase to induce site-specific protein-DNA nicks / Kristoffer P. Jakobsen and Lotte Bjergbaek -- A proximity ligation-based method for quantitative measurement of D-loop extension in S. cerevisiae / Aurèle Piazza, Romain Koszul, and Wolf-Dietrich Heyer -- Inducing and detecting mitotic DNA synthesis at difficult-to-replicate loci / Lorenza Garribba, Wei Wu, Özgün Özer, Rahul Bhowmick, Ian D. Hickson, and Ying Liu -- An approach to detect and study DNA double-strand break repair by transcript RNA using a spliced-antisense RNA template / Havva Keskin and Francesca Storici -- Assaying repair at DNA nicks / Luther Davis, Yinbo Zhang, and Nancy Maizels -- Recovery of alternative end-joining repair products from Drosophila embryos / Terrence Hanscom, Varandt Y. Khodaverdian, and Mitch McVey -- High-throughput analysis of DNA break-induced chromosome rearrangements by amplicon sequencing / Alexander J. Brown, Aneesa T. Al-Soodani, Miles Saul, Stephanie Her, Juan C. Garcia, Dale A. Ramsden, Chengtao Her, and Steven A. Roberts -- Assaying mutations associated with gene conversion repair of a double-strand break / Gajendrahar Dwivedi and James E. Haber -- Investigation of break-induced replication in yeast / Rajula Elango, Zachary Kockler, Liping Liu, and Anna Malkova -- Targeting allostery with avatars to design inhibitors assessed by cell activity: dissecting MRE11 endo- and exonuclease activities / Davide Moiani, Daryl A. Ronato, Chris A. Brosey, Andrew S. Arvai, Aleem Syed, Jean-Yves Masson, Elena Petricci, and John A Tainer -- Integrating experimental and in silico HTS in the discovery of inhibitors of protein-nucleic acid interactions / Quinn Li, Laura Folly da Silva Constantino, and M. Ashley Spies -- Monitoring recombination during meiosis in budding yeast / Shannon Owens, Shangming Tang, and Neil Hunter -- S1-seq assay for mapping processed DNA ends / Eleni P. Mimitou and Scott Keeney -- Methods for controlled protein depletion to study protein function during meiosis / Hardeep Kauer, Jasvinder S. Ahuja, and Michael Lichten -- Quantitative methods to investigate the 4D dynamics of heterochromatic repair sites in Drosophila cells / Christopher P. Caridi, Laetitia Delabaere, Harianto Tjong, Hannah Hopp, Devika Das, Frank Alber, and Irene Chiolo -- Analysis of meiotic double-strand break initiation in mammals / Kevin Brick, Florencia Pratto, Chi-Yu Sun, Rafael D. Camerini-Otero, and Galina Petukhova.