Part A: Chapter One: Physical Accuracy Leads to Biological Relevance: Best Practices For Simulating Ligand-Gated Ion Channels Interacting with General Anesthetics / Sruthi Murlidaran, Grace Brannigan -- Chapter Two: Computational Approaches to Studying Voltage-Gated Ion Channel Modulation by General Anesthetics / Eleonora Gianti, Vincenzo Carnevale -- Chapter Three: Molecular Mechanics Parameterization of Anesthetic Molecules / Thomas T. Joseph, Jérôme Hénin -- Chapter Four: Insights Into Receptor-Based Anesthetic Pharmacophores and Anesthetic-Protein Interactions / Victoria S. Fahrenbach, Edward J. Bertaccini -- Chapter Five: Understanding Anesthetic Mechanisms: Analysis of the Complex Kinetics of Ligand-Gated Ion Channels / Robert S. Cantor -- Chapter Six: General Genetic Strategies / Louise M. Steele, Philip G. Morgan -- Chapter Seven: Approaches to Anesthetic Mechanisms: The C. elegans Model / Louise M. Steele, Margaret M. Sedensky -- Chapter Eight: Using Drosophila to Understand General Anesthesia: From Synapses to Behavior / Shanker Karunanithi, Michael Troup, Bruno van Swinderen -- Chapter Nine: High-Throughput Screening to Identify Anesthetic Ligands Using Xenopus laevis Tadpoles / Kellie A. Woll, Roderic G. Eckenhoff -- Chapter Ten: Zebrafish: A Pharmacogenetic Model for Anesthesia / Victoria Bedell, Elena Buglo, Daniel Marcato, Christian Pylatiuk, Ralf Mikut, Johannes Stegmaier, Will Scudder, Maxwell Wray, Stephan Zuchner, Uwe Strahle, Ravindra Peravali, and Julia E. Dallman -- Chapter Eleven: The Mouse as a Model Organism for Assessing Anesthetic Sensitivity / Andrzej Z. Wasilczuk, Kaitlyn L. Maier, Max B. Kelz -- Chapter Twelve: Identification of General Anesthetic Target Protein-Binding Sites by Photoaffinity Labeling and Mass Spectrometry / Kellie A. Woll, William P. Dailey, Roderic G. Eckenhoff -- Chapter Thirteen: Xenon-Protein Interactions: Characterization by X-Ray Crystallography and Hyper-CEST NMR / Benjamin W. Roose, Serge D. Zemerov, Ivan J. Dmochowski -- Chapter Fourteen: Methods for Defining the Neuroprotective Properties of Xenon / Richie Robel, Peter Caroccio, Mervyn Maze -- Chapter Fifteen: Xenon Anesthesia and CT: Noninvasive Measures of Brain Anesthetic Concentration / Andrew McKinstry-Wu, Charles W. Carspecken, Alex Proekt, Max B. Kelz -- Chapter Sixteen: Native System and Cultured Cell Electrophysiology for Investigating Anesthetic Mechanisms / Paul Hoerbelt, Boris D. Heifets -- Chapter Seventeen: Electrophysiological Analysis of Voltage-Gated Ion Channel Modulation by General Anesthetics / Elaine Yang, Lianteng Zhi, Qiansheng Liang, Manuel Covarrubias -- Chapter Eighteen: Combining Mutations and Electrophysiology to Map Anesthetic Sites on Ligand-Gated Ion Channels / Stuart A. Forman -- Chapter Nineteen: HCN and K2P Channels in Anesthetic Mechanisms Research / Paul M. Riegelhaupt, Gareth R. Tibbs, Peter A. Goldstein.

Part B: Chapter One: Structural Analysis of Anesthetics in Complex with Soluble Proteins / Patrick J. Loll -- Chapter Two: X-Ray Crystallographic Studies for Revealing Binding Sites of General Anesthetics in Pentameric Ligand-Gated Ion Channels / Qiang Chen, Yan Xu, Pei Tang -- Chapter Three: Solution NMR Studies of Anesthetic Interactions with Ion Channels / Vasyl Bondarenko, Marta Wells, Yan Xu, Pei Tang -- Chapter Four: Time-Resolved Neutron Interferometry and the Mechanism of Electromechanical Coupling in Voltage-Gated Ion Channels / J. Kent Blasie -- Chapter Five: Fluorescent Anesthetics / Daniel J. Emerson, Ivan J. Dmochowski -- Chapter Six: Investigation of Anesthetic-Protein Interactions by a Thermodynamic Approach / Hasan Babazada, Renyu Liu -- Chapter Seven: Electron Spin Resonance (EPR) in Drosophila and General Anesthesia / Luca Turin, Efthimios M.C. Skoulakis -- Chapter Eight: Giant Plasma Membrane Vesicles: An Experimental Tool for Probing the Effects of Drugs and Other Conditions on Membrane Domain Stability / Zoe Gerstle, Rohan Desai, Sarah L. Veatch -- Chapter Nine: Anesthetic Drug Discovery and Development: A Case Study of Novel Etomidate Analogs / Megan McGrath, Douglas E. Raines -- Chapter Ten: Optoanesthesia: Use of Anesthetic Photolabels In Vivo / Andrew R. McKinstry-Wu, Max B. Kelz -- Chapter Eleven: Optogenetics and Chemogenetics / Ksenia Vlasov, Christa J. Van Dort, Ken Solt -- Chapter Twelve: Genetic Reporters of Neuronal Activity: c-Fos and G-CaMP6 / Andrew E. Hudson -- Chapter Thirteen: Genomics and Proteomic Techniques / Jonathan Z. Pan -- Chapter Fourteen: Neurochemistry of Anesthetic States / Ralph Lydic, Helen A. Baghdoyan, Amanda L. May -- Chapter Fifteen: Brief Introduction to Electroencephalography / Alex Proekt -- Chapter Sixteen: Using Positron Emission Tomography in Revealing the Mystery of General Anesthesia: Study Design Challenges and Opportunities / Harry Scheinin, Emilee C. Alkire, Annalotta Scheinin, Michael T. Alkire, Oskari Kantonen, and Jaakko Långsjö -- Chapter Seventeen: Brain Imaging Using Hyperpolarized 129Xe Magnetic Resonance Imaging / Simrun Chahal, Braedan R.J. Prete, Alanna Wade, Francis T. Hane, and Mitchell S. Albert.