Front Cover; Advances in Clinical Chemistry; Copyright; Contents; Contributors; Preface; Chapter One: Circulating Tumor Cell Isolation and Analysis; 1. Introduction; 1.1. CTCs as Liquid Biopsy; 1.2. Microfluidics Technology; 2. Methods of Isolation and Analysis; 2.1. Isolation Methods; 2.1.1. Isolation Based on Physical Properties; 2.1.1.1. Gradient Centrifugation; 2.1.1.2. Microfluidic Inertial Focusing; 2.1.1.3. Microfabricated Filters; 2.1.1.4. Dielectrophoresis; 2.1.1.5. Other Physical-Property-Based Methods; 2.1.2. Isolation Based on Biological Properties

2.1.2.1. Immunomagnetic Separation2.1.2.2. Microfluidics-Enabled Immunoseparation; 2.1.2.3. Other Biological-Property-Based Methods; 2.2. CTC Analysis; 2.2.1. Genetic Analysis of CTCs; 2.2.1.1. Quantitative Real-Time Polymerase Chain Reaction; 2.2.1.2. Fluorescent In Situ Hybridization; 2.2.1.3. Comparative Genomic Hybridization; 2.2.2. Protein and Functional Assays of CTCs; 2.2.2.1. Immunostaining; 2.2.2.2. EPISPOT Assay; 2.2.2.3. Invasion Assay; 2.2.3. Other Methods for CTC Analysis; 3. Modeling for CTC Studies; 3.1. Device Design Simulation; 3.2. CTC-Device Interaction Models

4. CTCs in Clinical Applications4.1. CTCs as Biomarkers; 4.1.1. Definition of Biomarkers; 4.1.2. Clinical Studies; 4.2. CTC Detection for Treatment Monitoring; 4.3. Potential Applications in Drug Discovery; 5. Conclusion; Acknowledgments; References; Chapter Two: Context and Applications of Targeted Genetic Testing, with Emphasis on Copy Number Variants; 1. Background; 2. Whole Genome Screen or Targeted Test?; 3. Methods; 3.1. Arrays and Software Targeting; 3.2. NGS and Software Filtering (Virtual Panels); 4. Results of Targeted Array CGH Tests for Prenatal Samples; 5. Discussion

6. ConclusionsReferences; Chapter Three: Circadian Genes in Breast Cancer; 1. Introduction; 2. Expression of Circadian Genes and Cancer; 2.1. Expression of Circadian Genes in Breast Cancer; 2.2. Methylation Status of Circadian Genes in Breast Cancer; 2.3. Circadian Genes and Shift Work; 3. Conclusions; Acknowledgments; References; Chapter Four: Transferrin Saturation: A Body Iron Biomarker; 1. Introduction; 2. Body Iron and Transferrin; 2.1. Body Iron: Distribution, Absorption, and Homeostasis; 2.2. Transferrin; 2.2.1. The Transferrin Family; 2.2.2. Human Serum Transferrin