Risk Management in Early Discovery Medicinal Chemistry / Gurpreet Singh, Jayme L. Dahlin and Michael A. Walters -- Target Identification Using Chemical Probes / Moses Moustakim, Suet Ling Felce, Nancy Zaarour, Gillian Farnie, Fiona E. McCann and Paul E. Brennan -- Mining the Microbiome for Drug Targets / Qi Wang, Fangming Yang and Huijue Jia -- Screening Library Design / Stephanie Kay Ashenden -- Design of a Fragment Screening Library / Ashley Taylor, Bradley C. Doak and Martin J. Scanlon -- Genetically Encoded Cyclic Peptide Libraries: From Hit to Lead and Beyond / Jacob Valentine and Ali Tavassoli -- A Guide to Run Affinity Screens Using Differential Scanning Fluorimetry and Surface Plasmon Resonance Assays / Christian Bergsdorf and S. Kirk Wright -- Second-Harmonic Generation (SHG) for Conformational Measurements: Assay Development, Optimization, and Screening / Tracy A. Young, Ben Moree, Margaret T. Butko, Bason Clancy, Mary Geck Do, Tarun Gheyi, John Strelow, Juan J. Carrillo and Joshua Salafsky -- An Array-Based Ligand Discovery Platform for Proteins With Short Half-Lives / Becky S. Leifer, Shelby K. Doyle, André Richters, Helen L. Evans and Angela N. Koehler -- High Content, Phenotypic Assays and Screens for Compounds Modulating Cellular Processes in Primary Neurons / Miklos Kepiro, Boglarka H. Varkuti and Ronald L. Davis -- Achieving a Good Crystal System for Crystallographic X-Ray Fragment Screening / Patrick M. Collins, Alice Douangamath, Romain Talon, Alexandre Dias, Jose Brandao-Neto, Tobias Krojer and Frank von Delft -- Hit-to-Lead: Hit Validation and Assessment / Kirk E. Hevener, Russell Pesavento, JinHong Ren, Hyun Lee, Kiira Ratia, and Michael E. Johnson -- Knowledge-Based Approaches to Off-Target Screening / Daniel R. McMasters.