I. Introduction. 1. Biology in the Computer Age. 2. Computational Approaches to Biological Questions -- II. The Bioinformatics Workstation. 3. Setting Up Your Workstation. 4. Files and Directories in Unix. 5. Working on a Unix System -- III. Tools for Bioinformatics. 6. Biological Research on the Web. 7. Sequence Analysis, Pairwise Alignment, and Database Searching. 8. Multiple Sequence Alignments, Trees, and Profiles. 9. Visualizing Protein Structures and Computing Structural Properties. 10. Predicting Protein Structure and Function from Sequence. 11. Tools for Genomics and Proteomics -- IV. Databases and Visualization. 12. Automating Data Analysis with Perl. 13. Building Biological Databases. 14. Visualization and Data Mining.