This 2nd edition of the Handbook "Regulatory Toxicology" was written and updated by experts for professionals, scientists and all those involved in toxicological data generation and decision-making. It includes a number of new chapters that cover recent developments, such as regulation of nanoparticles, illicit designer drugs or tattoos. The Handbook deals comprehensively with the safety-ensuring methods and concepts employed for acute and chronic toxicity by regulatory agencies, industry and academics.