1. Correlative Light and Electron Microscopy Analysis of the Centrosome; A Step by Step Protocol.<br>Dong Kong and Jadranka Loncarek<br>2.Generation of a Conditional Analog-Sensitive Kinase in Human Cells Using CRISPR/Cas9-Mediated Genome Engineering<br>Tyler Moyer and Andrew J. Holland<br>3. Studying Centrosome Function Using Three-Dimensional Cell Cultures<br>Teresa Arnandis and Susana A. Godinho<br>4. Analysis of Centrosomes in Human Cancer<br>Stefan Duensing<br>5. In Vivo Examination of the Cortical Cytoskeleton in Multiciliated Cells Using Electron Tomography<br>Daniel K. Clare, Maud Dumoux and Delphine Delacour<br>6.Tackling Centrosome Biology Through Gene Targeting in Chicken B Cells<br>Pavithra L. Chavali and Fanni Gergely<br>7. Centriole Biogenesis and Function in Multi-Ciliated Cells<br>Siwei Zhang and Brian J. Mitchell<br>8. Sub-Diffraction Resolution Microscopy Methods for Analyzing Centrosomes Organization<br>Vito Mennella, Rachel Hanna and Moshe Kim<br>9. Probing Mammalian Centrosome Structure Using Bioid Proximity-Dependent Biotinylation<br>Elif Nur Firat-Karalar and Tim Stearns<br>10. Purification of Centrosomes from Mammalian Cell Lines<br>Delphine Gogendeau, Paul Guichard and Anne-Marie Tassin<br>11. Isolation, Cryo-Tomography and Three-Dimensional Reconstruction of Centrioles<br>Paul Guichard, Virginie Hamel, Aitana Neves and Pierre Gönczy<br>12. New Insights into Centrosome Imaging in Drosophila and Mouse Neuroepithelial Tissues<br>Maria A. Rujano, Renata Basto and Véronique Marthiens <br>13. Microinjection Techniques for Studying Centrosome Function in Drosophila Melanogaster Syncytial Embryos<br>Paul T. Conduit, Daniel Hayward and James G. Wakefield<br>14. A Yeast Two-Hybrid Approach For Probing Protein-Protein Interactions at the Centrosome<br>Brian J. Galletta1 and Nasser M. Rusan<br>15. Genome-Wide RNAi Screens in S2 Cells To Identify Centrosome Components<br>Jeroen Dobbelaere<br>16. Time- Lapse Recording of Centrosomes and Other Organelles in Drosophila Neuroblasts<br>Judit Pampalona, Jens Januschke, Paula Sampaio, and Cayetano Gonzalez<br>17. Centrosomes and Spindles in Ascidian Embryos and Eggs<br>Alex McDougall, Janet Chenevert, Gerard Pruliere, Vlad Costache, Celine Hebras, Gregory Salez and Remi Dumollard<br>18. Ultrastructural Analysis of C. elegans Cilia<br>Daniel Serwas and Alexander Dammermann<br>19. Method: In Vitro Analysis of Pericentriolar Material Assembly<br>Jeffrey B. Woodruff and Anthony A. Hyman<br>20. Monitoring SPB Biogenesis in Fission Yeast with High Resolution and Quantitative Fluorescent Microscopy<br>Imène B. Bouhlel, Kathleen Scheffler, Phong T. Tran and Anne Paoletti