1. Biochemical Characterization of Function and Structure of RseP, an Escherichia coli S2P Protease / Y. Hizukuri, K. Akiyama, Y. Akiyama -- 2. Signal Peptidase Enzymology and Substrate Specificity Profiling / R.E. Dalbey, D. Pei, Ö.D. Ekici -- 3. Screening and Characterization Strategies for Nanobodies Targeting Membrane Proteins / S. Veugelen, M. Dewilde, B. De Strooper, L. Chávez-Gutiérrez -- 4. Probing the Activity of Eukaryotic Rhomboid Proteases In Vitro / B. Cordier, M.K. Lemberg -- 5. Expression, Purification, and Enzymatic Characterization of Intramembrane Proteases / R. Zhou, Y. Shi, G. Yang -- 6. Analyzing Amyloid-[beta] Peptide Modulation Profiles and Binding Sites of [gamma]-Secretase Modulators / J. Trambauer, A. Fukumori, B. Kretner, H. Steiner -- 7. Probing the Structure and Function Relationships of Presenilin by Substituted-Cysteine Accessibility Method / T. Tomita -- 8. A New Method to Determine the Transmembrane Conformation of Substrates in Intramembrane Proteolysis by Deep-UV Resonance Raman Spectroscopy / J.W. Cooley, A. Abdine, M. Brown, J. Chavez, B. Lada, R.D. JiJi, I. Ubarretxena-Belandia -- 9. An Inducible Reconstitution System for the Real-Time Kinetic Analysis of Protease Activity and Inhibition Inside the Membrane / R.P. Baker, S. Urban -- 10. Production of Recombinant Rhomboid Proteases / E. Arutyunova, R. Panigrahi, K. Strisovsky, M.J. Lemieux -- 11. Mechanism and Inhibition of Rhomboid Proteases / K. Strisovsky -- 12. Enzymatic Assays for Studying Intramembrane Proteolysis / D.M. Bolduc, D.J. Selkoe, M.S. Wolfe -- 13. Methods for Structural and Functional Analyses of Intramembrane Prenyltransferases in the UbiA Superfamily / Y. Yang, N. Ke, S. Liu, W. Li -- 14. Functional Study of the Vitamin K Cycle Enzymes in Live Cells / J.-K. Tie, D.W. Stafford -- 15. Activity Assays for Rhomboid Proteases / E. Arutyunova, K. Strisovsky, M.J. Lemieux.